Fig. 4. Cold stress induced lysine crotonylation of TaPGK.

(A) Immunoblots detected increased Kcr level of TaPGK in Fielder after cold treatment. IP, immunoprecipitation. (B) Immunoblots detected increased Kcr level of TaPGK in TaPGK-overexpressed wheat after cold treatment. (C) Immunoblots detected increased expression of TaPGK in TaPGK-overexpressed wheat after cold treatment. (D) Immunoblots detected decreased Kcr level of TaPGK^K206R but not TaPGK^K35R and TaPGK^K366R in transient expression tobacco. (E) In vivo detection results showed that TaPGK^K206R but not TaPGK^K35R and TaPGK^K366R had decreased enzymatic activity in transient expression tobacco. (F) Immunoblots detected decreased protein abundance of TaPGK^K206R in transient expression tobacco. (G) Immunoblots detected sharply increased Kcr level of TaPGK and but no visible change of Kcr level of TaPGK^206R in transient expression wheat leaf protoplasts after cold stress. Relative Kcr/TaPGK signals in the different genotypes are measured. The bands were quantified with ImageJ software. Bars are means ± SE from three measures of each of the two repeats. *P < 0.05 and **P < 0.01, as determined by paired Student’s t tests.