Figure 5. The effects of CR on glucose uptake do not differ between male and female mice.

Male and female C57BL6/NCrl mice were fed AL or CR diet from 9 to 15 weeks of age, as described for Figure 2. At 15 weeks of age, glucose uptake was assessed by PET/CT with ¹⁸F-fluorodeoxyglucose (¹⁸F-FDG). (A–C) ¹⁸F-FDG uptake in the indicated tissues was determined as SUVs from PET/CT scans (A) or as % injected dose per gram of tissue (%ID/g) from gamma counting of dissected whole tissues (C). (B) PET/CT images confirm that ¹⁸F-FDG uptake in interscapular BAT is greater in females than in males, regardless of diet. Data are shown as violin plots (A,C) or representative images (B) of 7 (male AL, female AL, male CR) or 6 (female CR) mice per group. For each tissue in (A) and (C), significant effects of sex, diet, and sex-diet interaction were assessed by two-way ANOVA; overall p values for each variable, and their interactions, are shown beneath each graph. Significant differences between each group, as determined using Tukey’s multiple comparisons test, are indicated by * (p<0.05). Source data are provided as a Source Data file. See also Figure 5—figure supplement 1.

Figure 5—figure supplement 1. The effects of CR on glucose uptake in peripheral tissues.

Male and female C57BL6/NCrl mice were fed AL or CR diet and glucose uptake was assessed by ¹⁸F-FDG PET/CT, as described for Figure 5. (A,B)¹⁸F-FDG uptake in the indicated tissues was determined by PMOD analysis of PET/CT scans (A) or gamma counting of dissected whole tissues (B). Data in (A) and (B) are presented as truncated violin plots, overlaid with individual data points, from 7 (male AL, female AL, male CR) or 6 (female CR) mice per group; in (B), data for spleen are from 6 mice per group only. Statistical significance was determined as described for Figure 5. Source data are provided as a Source Data file.