Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | 18F-Fluoro-deoxyglucose (18F-FDG) (radionuclide) | Edinburgh Clinical Research Imaging Centre (Edinburgh, UK) | N/A | |
| Chemical compound, drug | Ribozol | Amresco (USA) | N580 | |
| Commercial assay or kit | NEFA assay reagents | FUJIFILM Wako Chemicals Europe GmbH (Neuss, Germany) | 434–91795 436–91995 270–77000 |
|
| Antibody | anti-HSL (Rabbit polyclonal) | Cell Signaling Technology (Danvers, MA, USA) | #4107 RRID:AB_2296900 | (1:1000) |
| Antibody | Anti-Phospho-HSL (Ser563) (Rabbit polyclonal) | Cell Signaling Technology (Danvers, MA, USA) | #4139 RRID:AB_2135495 | (1:1000) |
| Antibody | Anti-beta actin (Rabbit polyclonal) | Abcam (Cambridge, UK) | #ab8227 RRID:AB_2305186 | (1:1000) |
| Antibody | IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody | LI-COR (Lincoln, NE, USA) | #926–32211 | (1:5000) |
| Strain, strain background (Mus musculus) | C57BL/6 J or C57BL/6NCrl | Charles River | 027 RRID:IMSR_CRL:027 | |
| Software, algorithm | Prism | GraphPad Software, LLC | v9.5.1 | |
| Software, algorithm | PMOD | PMOD Technologies LLC (Zurich, Switzerland) | v3.806 | |
| Software, algorithm | TrimGalore | https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ | V0.6.6 | |
| Software, algorithm | FastQC | https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ | v0.11.7 | |
| Software, algorithm | STAR | Dobin et al., 2013 | v2.7.10a | |
| Software, algorithm | Subread | Liao et al., 2014 | v1.5.2 | |
| Software, algorithm | R | https://www.r-project.org/ | v4.2.0 | |
| Software, algorithm | RStudio | https://posit.co/download/rstudio-desktop/ | v2022.12.0+353 | |
| Software, algorithm | DESeq2 | Love et al., 2014 | v1.36.0 | |
| Software, algorithm | GSEA | Mootha et al., 2003; Subramanian et al., 2005 | V4.3.2 | |
| Software, algorithm | Morpheus | https://software.broadinstitute.org/morpheus | NA | |
| Software, algorithm | Enricher | Xie et al., 2021; Kuleshov et al., 2016; Chen et al., 2013 | NA | |
| Software, algorithm | appyters | Clarke et al., 2021 | NA | |
| Software, algorithm | Image Studio | LI-COR Biosciences (USA) | v5.2 | |
| Software, algorithm | ExpeData | Sable Systems International (Las Vegas, USA) | v1.9.27 |