Table 1. Summary of CR protocol in each group of mice.

Because mice are singly housed, each mouse represents an independent experimental unit.

	Young	Aged	Evening-fed
Age at single housing	8 weeks	77 weeks	8 weeks
Age at start of CR	9 weeks	78 weeks	9 weeks
Time of feeding	0900–1000	0900–1000	1800–1900
Duration of CR	6 weeks	6 weeks	4 weeks
Related data	Figures 2—7, Figure 2—figure supplements 1–3; Figure 3—figure supplements 1–2; Figure 5—figure supplement 1; Figure 6—figure supplement 1; Figure 7—figure supplement 1; Figure 10—figure supplement 2	Figures 8–9; Figure 8—figure supplement 1; Figure 10—figure supplement 2	Figure 2—figure supplement 4; Figure 4—figure supplement 1
Experimental unit	Single mouse
Groups compared	AL vs CR (within sex); Male vs Female (within diet); and diet*sex interactions