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Confocal fluorescence microscopy images of A549 cells grown with 10 μM probe A under different hypoxia conditions in the absence and presence of 50, 100, and 150 μM CoCl2. The green channel I was used to obtain the visible fluorescence of probe A from 500 nm to 550 nm while red channel II was utilized to collect near-infrared fluorescence from 625 nm to 675 of probe A at 488 nm excitation. Scale bars of all images above are at 50 μm.
