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. 2023 May 3;38(13):959–973. doi: 10.1089/ars.2022.0058

FIG. 4.

FIG. 4.

Cytokine and chemokine expression in control and Keap1-KO CD4+ T cells under normoxic and hypoxic in vitro conditions. Control and Keap1-KO CD4+ T cells were cultured under normoxia or hypoxia for 72 h in vitro. (A) Significant Nrf2 target gene (Nqo1, Hmox1, Gclm) upregulation was confirmed under normoxia and hypoxia. There were no significant differences in Hif1a and Bcl2 between Keap1-KO and control cells under either condition. (B) Cytometric bead array data showed downregulation of IL2 in Keap1-KO CD4+ T cells compared with controls under normoxia and hypoxia. IL6 expression was significantly decreased in Keap1-KO CD4+ T cells under normoxia compared with controls. IFNγ upregulation was exacerbated under hypoxic conditions in Keap1-KO CD4+ T cells compared with control cells. For IL10, there were no significant changes between control and Keap1-KO cells in normoxic and hypoxic conditions. IL4, TNF-α, and IL17A expression was below detection level. n = 3 in each group. Statistical analyses were performed by paired, two-tailed T-test. *p < 0.05, **p < 0.001, ***p < 0.001, ****p < 0.0001. Bcl2, B-cell lymphoma 2 receptor; Hif1a, hypoxia-inducible factor 1 alpha.