. 2023 May 3;2023:8257217. doi: 10.1155/2023/8257217

Table 2.

Autophagy defects during hematopoietic differentiation in mice.

Model system	Target cells	Hematopoietic outcomes	Rf.
Fip200^flox/flox × Tie2-Cre	HSCs	Myeloproliferation, HSC apoptosis, and severe anemia.	[167]
Atg5^flox/flox × Vav-Cre		Lymphopenia, anemia, accumulation of monocytes, macrophages, and neutrophils.	[200]
Atg12^flox/flox × Mx1-cre		Increased apoptosis in HSCs and loss of HSCs.	[201]
Atg7^flox/flox × Vav-Cre		Loss of HSC function, severe myeloproliferation, defective megakaryocyte homeostasis, platelet aggregation, apoptosis in bone marrow, defective HSPC quiescence, and cell cycle arrest.	[165, 202, 203]

Atg5^flox/flox × CD19-Cre	B cells	Defective antibody responses in B cells, increased cell death in BM, depletion in B-1 B cells, defective B cell homeostasis, EMH, and anemia.	[183, 184, 187]
Atg5^flox/− × CD21-Cre	B cells	Decreased T1 B cells and follicular B-cell numbers, reduced B-1a and B-2 B-cell proportion.	[185]

Atg5^flox/flox × Gzmb-Cre Atg7^flox/flox × Gzmb-Cre	T cells	Pathogen infection, loss of memory T cell function.	[197]
Atg7^flox/flox × CD4-Cre		Loss of iNKT in lymphoid organs, lymphopenia, severely compromised CD8⁺ memory T cells.	[192]
Atg3^flox/flox × Lck-Cre		Decreased T cell numbers.	[195]
Atg5^flox/flox × Cre-ER^T2		Loss of CD8⁺ T cells, a severe reduction in lymphoid-specific memory T cells.	[198]
Atg5^flox/flox × CD4-Cre, Atg7^flox/flox × Lck-Cre		Significant reduction in iNKT, CD4, and CD8 T cell numbers.	[193, 194]
Vps34^flox/flox;CD4-Cre		Impaired T cell homeostasis and anemia.	[189]
Atg7^flox/flox;CD4-Cre		Progressive anemia perturbed iNKT cell development.	[191]
Atg16l1^flox/flox;Cd11c-^Cre		GVHD with increased T cell proliferation.	[199]
Atg16l1^flox/flox;CD4-Cre		Expanded T cell proliferation, impaired innate NK T lymphocyte development.	[190]

Atg3^flox/flox Ubc;cre-ERT2	NK cells	Loss of memory NK cells.	[204]
Atg5^flox/flox;NKp46-Cre	NK cells	Impairment in NK cell development, reduction in iNKs and mNKs in the spleen and BM.	[205]

ATG5^flox/flox-Lyz-Cre	Macrophages	Perturbed lymphoid and myeloid cell homeostasis, altered macrophage differentiation.	[206]

Atg7^Flox/Flox; Mx-Cre	Mast cells	Impairment of mast cell degranulation.	[207]

Vav-Cre × Atg7^flox/flox Cebpa-cre × Atg7^flox/flox Mx1-cre × Atg5^flox/flox	Neutrophils and Eosinophils	Impaired neutrophil differentiation.	[208]
Atg7^flox/flox;Lyz2-Cr		Eosinophilic inflammation.	[209]
Atg7^flox/flox;LysM-cre		Reduced neutrophil degranulation, increased circulating neutrophil numbers, decreased inflammatory potential of neutrophils.	[210]

Atg5^flox/flox;CD11c-Cre	Dendritic cells	Reduced migration of DCs.	[211]

Atg7^flox/flox;PF4-Cre	Megakaryocytes and Platelets	Impaired thrombosis, robust bleeding, and platelet aggregation.	[212]
VPS34^flox/flox;PF4-Cre		Impeded thrombus formation.	[213]
Atg5^flox/flox;PF4-Cre		Delayed thrombus formation, pulmonary thrombosis, and significantly reduced platelet aggregation.	[214]

mNKs: mature natural killers; BM: bone marrow; iNKT: invariant natural killer T: EMH: extramedullary hematopoiesis; CD: cluster of differentiation; DCs: dendritic cells.