Fig. 1.

Replication, subcellular localization, and systematic responses following simultaneous DENV2/ZIKV co-infection of C6/36 mosquito cells. A Immunofluorescence of the flaviviral NS1 (green) in ZIKV single-infected and DENV2/ZIKV co-infected cells, with DAPI (blue)-stained DNA demarcating nuclei. Scale bars, 10 μm. B Relative levels of DENV and ZIKV viral genomes in cell lysate at 2 days post-infection (dpi). C Relative expression of genes encoding catalase and glutathione S-transferase family proteins in cell lysate at 2 dpi. These results are representative of at least three independent experiments. D Relative expression of casp7, dronc, and Mx in cell lysate at 2 dpi. These results are representative of at least three independent experiments. Differences between groups were demonstrated to be statistically significant using Tukey’s multiple comparisons test; *P < 0.05, **P < 0.01, ***P < 0.001