Table 1. qRT-PCR primers.
Gene | Primer sequences (5′-3′) |
GCLC | F: ACAGCACGTTGCTCATCTCT |
R: TGGTCAGACTCGTTGGCATC | |
HO-1 | F: GCCTGGTTCAAGATACTACCTCT |
R: CTGAGTGTGAGGACCCATCG | |
Caspase-3 | F: GAGCTTGGAACGCGAAGAAA |
R: GAGTCCATCGACTTGCTTCCA | |
Bcl-2 | F: GGTGAACTGGGGGAGGATTG |
R: AGAGCGATGTTGTCCACCAG | |
Bax | F: ACGTCTGCGGGGAGTCAC |
R: AGGCCCCTGTCTTCATGATCT |
qRT-PCR: Quantitative real-time polymerase chain reaction; HO-1: Heme oxygenase-1; GCLC: Glutamate-cysteine ligase catalytic subunit.