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. 2023 Apr 3;7(5):768–781. doi: 10.1038/s41559-023-02027-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, Division of genera in social specialists (SNB < 0.35) and social generalists (other). Arrows represent the direction of the GSEA in panels b and c. b, GSEA on the pan-genome of all genera sorted by SNB, on the level of subsystem subclasses. c, GSEA on the pan-genome of specialist genera sorted by mean α diversity of the samples in which they are found, on the level of subsystem subclasses. Enriched functions (FDR < 0.1) in b and c are sorted according to normalized enrichment score (NES). The size of the bars indicates the total number of genera in the dataset having the function. The most enriched functions are in the upper and lower parts of the panels. Full GSEA information, including analyses of other functional universes (subsystem names, gene ontologies and pathways), is available in Supplementary Data 8 and 9.