Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 10;14:2692. doi: 10.1038/s41467-023-38360-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Pathologist-annotated regions of cancer tissue section from patient “No.0602”. b Visium array spots colored by graph-based clustering algorithm. c UMAP plot of gastric cancer tissue colored by clusters. d Cell cluster annotation of gastric cancer tissue. e The fusion image of visium array spots and H&E stain image, scale bar = 2 mm. The H&E stain experiment was repeated three times. f, g MS images of glutamine and glutamate in whole gastric cancer tissue section and in lymphoid tissue regions, intensity in colour scale is relative value. h Spatial expression images of GLS in whole gastric cancer tissue section and in lymphoid tissue regions, intensity in colour scale is log2 transformed. i–l Expression levels of glutamine, glutamate, GLS genes and SLC1A5 in different gastric cancer tissue section spots (seven tissue samples for spatial metabolomics, n = 6 independent section regions from patient “No.0602”, mean ± SD), p-values were calculated using the unpaired two-tailed t-test at confidence intervals 0.95. m–p MS images and expression levels of arachidonic acid, docosahexaenoic acid, docosapentaenoic acid, and docosatetraenoic acid in different gastric cancer tissue section spots (seven tissue samples for spatial lipidomics, n = 6 independent section regions per tissue sample, mean ± SD), intensity in colour scale is relative value. q–t Spatial images and expression levels of FASN, SCD, ELOVL1, and ALOX5AP in gastric cancer tissue, intensity in colour scale is log2 transformed. TT Tumor tissue, SGS Serrated glandular structure, NE Normal epithelium, LT Lymphoid tissue, PLT Peritumoral lymphoid tissue, MM Muscularis mucosa, MT Muscle tissue, CNT Connective tissue.