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Down-regulation of OspA in HAB. (A) RT-PCR of ospA and flaB from 107 IVCB (Bb) versus HAB. Rabbit actin was used as a control. (B) Western immunoblot of rOspA using basal sera (lane B) or serum 21 days postinfection (lane 21) from rabbits infected either by needle inoculation of ∼108 IVCB (In vitro) or by implantation of ∼108 HAB from EM lesion skin biopsies. Markers to the left are in kilodaltons.
