TABLE 1.
EM and culture results of challenge with IVCB and HAB
| Immunization | Challengea | EM lesionsb (no. positive/ no. tested) | No. of rabbits with positive cultures/ no. in group
|
Symptom scored | ||
|---|---|---|---|---|---|---|
| Skin
|
Viscerac 3 wk | |||||
| 8 day | 3 wk | |||||
| rOspA | IVCB | 5/5 | 1/5 | 4/5 | 2/5 | 12/20 |
| Avirulent B31 OMV | IVCB | 1/5 | 3/5 | 2/5 | 1/5 | 7/20 |
| Virulent B31 OMV | IVCB | 0/5 | 1/5 | 0/5 | 0/5 | 1/20 |
| B313 OMV | IVCB | 0/5 | 2/5 | 0/5 | 0/5 | 2/20 |
| Infectione | IVCB | 0/3 | 0/3 | 0/3 | 0/3 | 0/12 |
| Naive | IVCB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
| rOspA | HAB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
| Avirulent B31 OMV | HAB | 3/5 | 5/5 | 5/5 | 5/5 | 18/20 |
| Virulent B31 OMV | HAB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
| B313 OMV | HAB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
| Infectione | HAB | 0/3 | 0/3 | 0/3 | 0/3 | 0/12 |
| Naive | HAB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
a
Rabbits were challenged by intradermal inoculation with 6 × 104 IVCB or by implantation of EM skin biopsies containing approximately 8.1 × 106 to 1.4 × 108 HAB.
b
EM lesions appeared 6 to 13 days postchallenge; rabbits were scored positive if any of the six sites developed EM lesions.
c
Viscera were scored positive if any of the joints, popliteal lymph nodes, spinal cord, or spleen tissues were culture positive.
d
Total number of symptoms of EM, 8-day skin, and 3-week skin and visceral cultures/total possible score.
e
Infection-derived immunity.