TABLE 1.
Immunization | Challengea | EM lesionsb (no. positive/ no. tested) | No. of rabbits with positive cultures/ no. in group
|
Symptom scored | ||
---|---|---|---|---|---|---|
Skin
|
Viscerac 3 wk | |||||
8 day | 3 wk | |||||
rOspA | IVCB | 5/5 | 1/5 | 4/5 | 2/5 | 12/20 |
Avirulent B31 OMV | IVCB | 1/5 | 3/5 | 2/5 | 1/5 | 7/20 |
Virulent B31 OMV | IVCB | 0/5 | 1/5 | 0/5 | 0/5 | 1/20 |
B313 OMV | IVCB | 0/5 | 2/5 | 0/5 | 0/5 | 2/20 |
Infectione | IVCB | 0/3 | 0/3 | 0/3 | 0/3 | 0/12 |
Naive | IVCB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
rOspA | HAB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
Avirulent B31 OMV | HAB | 3/5 | 5/5 | 5/5 | 5/5 | 18/20 |
Virulent B31 OMV | HAB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
B313 OMV | HAB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
Infectione | HAB | 0/3 | 0/3 | 0/3 | 0/3 | 0/12 |
Naive | HAB | 5/5 | 5/5 | 5/5 | 5/5 | 20/20 |
Rabbits were challenged by intradermal inoculation with 6 × 104 IVCB or by implantation of EM skin biopsies containing approximately 8.1 × 106 to 1.4 × 108 HAB.
EM lesions appeared 6 to 13 days postchallenge; rabbits were scored positive if any of the six sites developed EM lesions.
Viscera were scored positive if any of the joints, popliteal lymph nodes, spinal cord, or spleen tissues were culture positive.
Total number of symptoms of EM, 8-day skin, and 3-week skin and visceral cultures/total possible score.
Infection-derived immunity.