Skip to main content
. 2023 May 10;13:7604. doi: 10.1038/s41598-023-34440-0

Figure 1.

Figure 1

Identification of PP compounds as CFTR correctors. (A) Screening of a focused chemical library. The graph shows results obtained with the HS-YFP assay on CFBE41o- cells expressing F508del-CFTR and treated with test compounds (10 µM) for 24 h. Each circle reports CFTR activity (quenching rate, QR) for a single compound. Dotted lines show average activity of negative (vehicle) and positive (VX-809, 1 µM) controls. PP007 was active as a corrector. (B) Representative HS-YFP assay traces (left) and summary of data (right) revealing synergy of PP007 with VX-809. Quenching of HS-YFP was induced by extracellular addition of I–rich solution (arrow). *, p < 0.05; ***, p < 0.001; vs. vehicle. #, p < 0.05; ###, p < 0.001; vs. VX-809 alone (ANOVA with Tukey’s post-hoc test). (C) Activity data (HS-YFP assay) for indicated compounds, tested at 1 and 10 µM plus/minus VX-809 (1 µM). PP008 and PP011 are effective correctors. **, p < 0.01; ***, p < 0.001; vs. vehicle. ##, p < 0.01; ###, p < 0.001; vs. VX-809 alone (ANOVA with Tukey’s post-hoc test).