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. 2023 Feb 2;63(1):56–64. doi: 10.1007/s12088-023-01056-x

Fig. 5.

Fig. 5

Comparison of Putrescine monooxygenase expression level in Lon knockout E. coliK-12 and wild type E. coliK-12 cells containing plasmid pET151TOPOSpPMO Lane1: Un-induced knockout transformant, Lane2: Induced knockout transformant for 3 h at 37℃, Lane3: Induced knockout transformant for 4 h at 37℃, Lane M: Dual colour precision plus protein standards, Lane5: Un-induced wild-type transformant, Lane6: Induced wild-type transformant for 3 h at 37℃, Lane7: Induced wild-type transformant for 4 h at 37℃