Fig. 5.
Comparison of Putrescine monooxygenase expression level in Lon knockout E. coliK-12 and wild type E. coliK-12 cells containing plasmid pET151TOPOSpPMO Lane1: Un-induced knockout transformant, Lane2: Induced knockout transformant for 3 h at 37℃, Lane3: Induced knockout transformant for 4 h at 37℃, Lane M: Dual colour precision plus protein standards, Lane5: Un-induced wild-type transformant, Lane6: Induced wild-type transformant for 3 h at 37℃, Lane7: Induced wild-type transformant for 4 h at 37℃