Table 1.
Strains and plasmids used in this study
| Strain/Plasmids | Genotype | Source |
|---|---|---|
| Escherichia coli | ||
| BL21 Star(DE3) | F– ompT hsdSB (rB–, mB–) gal dcm rne131 (DE3) | Invitrogen |
| DH5α | F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Invitrogen |
| K-12 | FΔ(HindIII)::cat (Tra+ Pil+ CamR)/ ung-1 relA1 dut-1 thi-1 spoT1 mcrA | In-house |
| Plasmids | ||
| pET28a(Cre) | The plasmid carrying Cre- Kanr CORE cassette | Our study |
| pColdIV(Cre) | The plasmid carrying Cre- Ampr CORE cassette | Our study |
| pET151TOPOSpPMO | The plasmid carrying PMO gene-Ampr | Our study |