TABLE 3.
Immunohistochemical analysis of inclusion bodies in hormone-treated rats
| Groupa | Tissue | No. of IBb | Overall histologic assessment |
|---|---|---|---|
| S | Uterus | 3 | Mild infection with inflammation |
| Vagina | 28 | Extensive infection with inflammation | |
| E | Uterus | 0 | No infection, no inflammation |
| Vagina | 0 | No infection, no inflammation | |
| P | Uterus | 8 | Moderate infection with inflammation |
| Vagina | 30 | Extensive infection with inflammation | |
| E+P | Uterus | 7 | Faint staining, no inflammation |
| Vagina | 48 | Extensive focal infection, no inflammation |
a
Experimental groups consisted of ovariectomized rats treated with saline (S), estradiol (E), progesterone (P), or estradiol and progesterone (E+P).
b
Total number of inclusion bodies (IB) counted on each slide stained with anti-Chlamydia antibody, as described in Materials and Methods. Each slide had at least one representative tissue section from each animal (n = 4).