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Induction of SAP in the serum of IL-1β-desensitized or hTNF-α-tolerized mice. SAP was determined by ELISA and is expressed as a percentage of the control (PBS-treated mice = 100%). A total of 0.3 μg of IL-1β was injected i.p., and SAP was measured 12 h later. hTNF-α was injected twice per day for 5 days, and SAP was measured 3 days later. ∗, P = 0.001; ∗∗, P < 0.001 (n = 6 in all groups).
