TABLE 2.
IL-1β desensitizes SAP+/+ as well as SAP0/0 mice to TNF-α–GalN-induced lethality
| Pretreatmenta | Lethalityb (no. of animals killed/total no.)
|
|
|---|---|---|
| SAP+/+ (P) | SAP0/0 (P) | |
| PBS | 10/12 | 7/11 |
| 3 ng of IL-1β | 6/7 (NS) | 4/6 (NS) |
| 30 ng of IL-1β | 2/7** (0.0085) | 3/5 (NS) |
| 300 ng of IL-1β | 0/6** (0.0004) | 0/6* (0.0054) |
a
Pretreatment was given i.p. 12 h before the challenge.
b
Challenge was 0.5 μg of mTNF-α plus 20 mg of GalN given i.p. (doses are per 20 g of body weight). Lethality was scored for up to 48 h (no further deaths occurred). NS, not significantly different from PBS-pretreated mice. ∗ and ∗∗, P < 0.01 and P < 0.001, respectively, versus PBS-pretreated mice.