Table 1.
Intraassay and intermouse variations of the mouse WB-TG assay
| Intraassay CV (n = 5 replicates) | ||||||
|---|---|---|---|---|---|---|
| Silica |
0.05 pM TF |
|||||
| Mean | SD | %CV | Mean | SD | %CV | |
| Lag time (min) | 10.7 | 0.4 | 3.7 | 9.3 | 0.1 | 1.4 |
| TtPeak (min) | 12.9 | 0.5 | 4.2 | 13.6 | 1.2 | 8.6 |
| Peak height (nM) | 71.8 | 6.8 | 9.4 | 40.6 | 3.9 | 9.7 |
| ETP (nM∗min) | 833.0 | 66.7 | 8.0 | 839.1 | 39.7 | 4.7 |
| Intermouse CV (n = 6 mice) | ||||||
|---|---|---|---|---|---|---|
| Silica |
0.05 pM TF |
|||||
| Mean | SD | %CV | Mean | SD | %CV | |
| Lag time (min) | 12.4 | 1.5 | 11.8 | 8.5 | 0.5 | 5.9 |
| TtPeak (min) | 15.7 | 1.4 | 8.9 | 12.6 | 0.7 | 5.5 |
| Peak height (nM) | 56.0 | 6.9 | 12.3 | 50.0 | 6.9 | 13.7 |
| ETP (nM∗min) | 810.3 | 55.2 | 6.8 | 850.0 | 40.9 | 4.8 |
The intraassay variation was determined by measuring TG in mouse WB collected in citrate and 50 μg/mL CTI triggered by 9 mM CaCl2 and either 0.05 pM TF or 120× diluted silica in the presence of 416.7 μM thrombin substrate ZGGR-AMC. The intermouse variations of WB-TG parameters were assessed in 6 mice done in 2 independent runs.
CV, coefficient of variation; SD, standard deviation; TtPeak, time to peak.