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Western blot of sIgA antibodies to EPEC (strain E2348/69) in human milk. Lanes 1 and 2, whole-cell extracts probed with pooled human milk diluted at a ratio of 1:10 (lane 1) and 1:200 (lane 2); lanes 3 and 4, bacterial surface and outer-membrane extracts probed with human milk (1:200); lane 5, culture supernatant proteins probed with human milk (1:200).
