Fig. 5.

Brillouin optical microscopy and its corneal imaging reproduced from reference [173]. (a) Schematic of a Brillouin microscopy set-up. (b) A typical CCD output from the spectrometer displaying the Brillouin spectrum of the corneal stroma. (c) Brillouin spectrum (red trace) analysis using Lorentzian curve fit (gray trace). (d) Cross-sectional Brillouin image of the ex vivo bovine cornea. The Brillouin shift is a decrease in depth, revealing the depth-decreased longitudinal modulus. The horizontal (x) and vertical (z) span is 5 × 0.5 mm. Scale bars: 200 µm. (e) and (f) En face Brillouin image of the cornea at different depths. Scale bars: 1 mm.