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. 2023 Feb 16;29(6):1602–1614. doi: 10.1111/cns.14123

FIGURE 2.

FIGURE 2

SLD lesion eliminates REM sleep in rat. To investigate whether the SLD is necessary for REM sleep, we ablated SLD neurons by bilateral injections of AAV‐DTA and examined sleep–wake parameters in lesioned vs. control rats. We obtained partial lesion group (N = 5), complete lesion group (N = 5), and control group (N = 7). Hourly wake, REM sleep, and NREM sleep amounts across 24 h are shown in A, C, and E while total amounts of wake, REM sleep, and NREM sleep in 24, 12 dark and 12 h light period are shown in B, D, and F. Histology of SLD location, partial lesion, and complete lesion are shown in G, H, and I. Complete SLD lesions eliminate REM sleep, significantly reduce NREM sleep during the light period, and significantly increase wake amounts in 24 h and light period. Partial SLD lesions have less effect on sleep–wake than complete SLD lesions, but produce RBD‐like movements (Figure S3). Hourly wake, REM, and NREM sleep amounts were analyzed using two‐way ANOVA, and total amounts of each stage were analyzed using one‐way ANOVA, followed by Bonferroni's post hoc test, *p < 0.05, **p < 0.01. Scale bar = 200 μm.