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. 2023 Feb 16;29(6):1537–1546. doi: 10.1111/cns.14116

FIGURE 1.

FIGURE 1

Effect of folic acid deficiency on GFAP expression in astrocytes. (A, B) Double labeling immunofluorescence of DAPI (blue) and GFAP (red) in SHAM, MCAO, and MCAO+FD group rats after 12 h and 24 h of reperfusion. Data shown are mean ± SEM (n = 4). a p <0.05: Compared to SHAM, b p <0.05: Compared to MCAO 12 h, c p <0.05: Compared to MCAO+FD 12 h. The cells were harvested after incubating with normal DMEM, normal DMEM and OGD/R, folic acid deficient DMEM and OGD/R. (C, D) Western blot analyses of GFAP and β‐Actin. Data shown are mean ± SEM (n = 4). a p <0.05: Compared to Control.