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. 2023 Feb 16;29(6):1537–1546. doi: 10.1111/cns.14116

FIGURE 4.

FIGURE 4

Folic acid deficiency regulates the expression of pSTAT3 in primary astrocytes exposed to hypoxia and glucose deficiency via the JAK‐1 pathway. (A‐E) The cells were harvested after incubating with Filgotinib (JAK‐1 inhibitor). The protein expressions of IL‐6 (A), pSTAT3, and STAT3 (B) were detected by western blot. Bar graphs show the relative levels of IL‐6 (normalized to β‐Actin) (C), pSTAT3 (normalized to STAT3) (D), and STAT3 (normalized to β‐Actin) (E). (F‐J) The cells were harvested after incubating with AG490 (JAK‐2 inhibitor). The protein expressions of IL‐6 (F), pSTAT3 and STAT3 (G) were detected by western blot. Bar graphs show the relative level of IL‐6 (normalized to β‐Actin) (H), pSTAT3 (normalized to STAT3) (I) and STAT3 (normalized to β‐Actin) (J). Data are shown as mean ± SEM (n = 4). a p <0.05: Compared to OGD/R group. b p <0.05: Compared to FD + OGD/R group.