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. 2023 Apr 14;26(5):106676. doi: 10.1016/j.isci.2023.106676

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© 2023 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Phylogenetic analysis of PbAMTs 1–6

(A) Domain structure of PbAMTs 1–6. MT-A70 domains of PbAMTs 1–4 are predicted by CD-Search, while that of PbAMT6 is inferred from sequence alignment with PbAMTs 1–4. The structure of AMTs 1–7 of T. thermophila (AMT2 is also referred to as TAMT-1) is also shown for comparison. MT-A70 domain, DPPW, and GNEL motif are highlighted by purple, red, and green, respectively.

(B) Phylogenetic analysis of MT-A70 proteins. Putative DNA 6mA (subclades AMT1, AMT2/5, AMT6/7, and METTL4/DAMT1) and RNA m6A (subclades METTL3 and METTL14) methyltransferase candidates are separated by a dashed curve. The eukaryotic tree (top left panel) is shown with different colors according to their phylogenetic position. PbAMTs 1–6 of P. bursaria are shown in bold plus cyan in different clades. The scale bar is 0.5. Species full name and NCBI GI number are shown in Table S13.