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. 2023 May 10;55(1):2210845. doi: 10.1080/07853890.2023.2210845

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 7. — Flow Immune cells in the spleen were analysed using cytometry. (A) Illustration of a gating method for detecting CD3, CD4 and CD8 in spleens. (B) Percentage of CD3, CD4 and CD8 in spleens. (C) A typical gating technique for detecting M2-TAM and Granulocytic MDSC in spleens. (D) Neutrophil count in spleens expressed as a proportion of viable cells. (E) M2-TAM in spleens was estimated as a proportion of viable cells. Three trials with six mice per group were demonstrated with representative data. GraphPad Prism was used to analyse the data. Data were represented by mean ± SEM, *p < .05, **p < .01 and ***p < .001.