Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Denaturation buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris (99%) | 38% | 19 g |
| NaCl 5 M | 200 mM | 4 mL |
| SDS 350 mM | 200 mM | 57,14 mL |
| Adjust pH to 9 with HCl | ||
| ddH2O | N/A | ∼25 mL |
| Total | N/A | 100 mL |
Filter (0.45 μm) and keep at RT. Place at 37°C for 10 min if crystals form.
