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. Author manuscript; available in PMC: 2023 May 11.
Published in final edited form as: Toxicol Lett. 2022 Jul 25;368:9–23. doi: 10.1016/j.toxlet.2022.07.009

Figure 3. Effect of AFPs on HUH7 hepatocyte Viability and proliferation.

Figure 3.

Cell viability was determined in HUH7 cells treated with vehicle (0) or up to 1000 μg/mL of DAFP1 (A-D) or TmAFP (E-F) for 48h or 72h by MTT assay. Proliferation was measured by EDU incorporation assay, with representative images of EDU incorporation shown in C (scale in μm.), and quantification shown in D. Results represent the means ± SEM of 3 independent experiments conducted each in triplicates and are presented as fold change of vehicle control (0 μg/mL). Significant differences relative to vehicle control were calculated with One way ANOVA test and multiple comparisons or t-test: * p≤0.05, ** p≤0.01, *** p≤0.001.