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. Author manuscript; available in PMC: 2023 May 11.
Published in final edited form as: Toxicol Lett. 2022 Jul 25;368:9–23. doi: 10.1016/j.toxlet.2022.07.009

Figure 6. Effect of DAFP1 on the expression of inflammatory genes and TNFa cytokine in RAW 264.7 macrophages.

Figure 6.

Effect of 40h treatment with vehicle or 1000 μg/mL DAFP1 on (A-D) gene expression of the inflammatory genes Il1b, Cxcl2, Tnfa, and Cxcl10 measured by qPCR analysis, normalized to HPRT. (E) Effect of 40h treatment with vehicle or 1000 μg/mL DAFP1 on TNFa secreted protein measured by ELISA assay. Results are normalized to total RNA content in cells. N=3 independent experiments conducted in triplicates. Significant difference relative to vehicle control with Student t-test: *** p≤0.001.