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. 2023 Apr 8;43(5):562–581. doi: 10.1002/cac2.12420

FIGURE 5.

FIGURE 5

HTB promotes the formation of PD‐L1+ M2 macrophages, (A) Flow cytometry revealed the proportion of apoptotic Mac‐Ps after exposure to control medium (CM, 50% complete medium + 50% serum free medium) or LTB MC38 subcutaneous tumor tissue culture supernatants (LTCS) or HTB MC38 subcutaneous tumor tissue culture supernatants (HTCS) for 24 hours (n = 3), (B) Flow cytometry revealed the proportion of apoptotic Mac‐Ps after exposure to control medium (CM) or LTB B16 subcutaneous tumor tissue culture supernatants (LTCS) or HTB B16 subcutaneous tumor tissue culture supernatants (HTCS) for 24 h (n = 3), (C) Flow cytometry revealed the proportion of M2 Mac‐Ps after exposure to CM or MC38 subcutaneous tumor tissue culture supernatants for 24 h (n = 3), (D) Flow cytometry revealed the proportion of M2 Mac‐Ps after exposure to CM or B16 subcutaneous tumor tissue culture supernatants for 24 h (n = 3), (E) Flow cytometry revealed the proportion of PD‐L1+ M2 Mac‐Ps after exposure to CM or MC38 subcutaneous tumor tissue culture supernatants for 24 h (n = 3), (F) Flow cytometry revealed the proportion of PD‐L1+ M2 Mac‐Ps after exposure to CM or B16 subcutaneous tumor tissue culture supernatants for 24 h (n = 3). ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001. Abbreviations: LTB, low tumor burden; HTB, high tumor burden; PD‐L1, programmed death‐ligand 1; mac‐P, peritoneal macrophage; CM, control medium; LTCS, low tumor burden subcutaneous tumor tissue culture supernatants (50% complete medium + 50% MC38 or B16 low tumor burden subcutaneous tumor tissue culture supernatants); HTCS, high tumor burden subcutaneous tumor tissue culture supernatants (50% complete medium + 50% MC38 or B16 high tumor burden subcutaneous tumor tissue culture supernatants).