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. Author manuscript; available in PMC: 2023 May 11.
Published in final edited form as: Nat Cell Biol. 2022 Oct 6;24(10):1516–1527. doi: 10.1038/s41556-022-00992-y

Extended Figure 6. Cleaving RAD21 in NBS2 dissociates and releases cohesin components.

Extended Figure 6

(a) Western blot analysis of salt effects on nuclear retention of cohesin complex subunits in HAP-1RAD21TEV nuclei treated with TEV as shown. Western blot analysis was performed as indicated in Extended Data Fig. 4ab except that 200mM NaCl was used, instead of 132mM. (b) Quantification of levels of SMC1/3, N-terminal cleaved RAD21 and C-terminal cleaved RAD21. The levels of indicated proteins or fragments were normalized to LMNA as loading controls first, then the ratio was normalized to the same protein or fragments in NB without TEV treatment. For SMC proteins, the average relative percentage of SMC1 and SMC3 levels, from panel (a) and Fig. 3a, is presented. See source data for numerical data and unprocessed blots.