FIG. 2.

CSF mRNA expression following exposure of mouse osteoblasts to viable or UV-killed S. aureus. Murine osteoblasts were left unexposed (lanes 0) or exposed to viable or UV-killed S. aureus at the indicated ratio (250:1, 75:1, or 25:1 bacterium-to-osteoblast ratio) for 45 min, and then extracellular bacteria were removed. RNA was isolated 6 and 12 h following exposure to bacteria, and a semiquantitative RT-PCR was performed for each mRNA species. Results are presented as amplified products electrophoresed on ethidium bromide-stained agarose gels. RT-PCR amplification of the G3PDH housekeeping gene was performed to ensure that similar amounts of input RNA and similar RT efficiencies were being compared. These studies were performed three times with similar results.