FIG. 5.

Dose-dependent Salmonella induction of CSF secretion by cultured mouse osteoblasts. Osteoblasts (10⁷) were exposed to various numbers of viable or UV-killed Salmonella bacteria (30:1, 10:1, and 3:1 bacterium-to-osteoblast ratios) for 45 min. Extracellular bacteria were then eliminated, and culture supernatants were taken 24 and 48 h postexposure. Specific capture ELISAs were performed to quantify CSF secretion. Results are presented as means of triplicate determinations ± standard deviations. These studies were performed three times with similar results.