FIG. 6.

Dose-dependent S. aureus (Staph) induction of CSF secretion by cultured human osteoblasts. Osteoblasts (3 × 10⁶) were exposed to various numbers of viable or UV-killed S. aureus bacteria (250:1, 75:1, and 25:1 bacterium-to-osteoblast ratios) for 45 min. Extracellular bacteria were then eliminated, and culture supernatants were taken 24 and 48 h postexposure. Specific capture ELISAs were performed to quantify CSF secretion. Results are presented as means of triplicate determinations ± standard deviations. These studies were performed twice with similar results.