FIG. 2.

Immunoblot analysis of patient serum reactivity against recombinant EHEC virulence proteins. (A) Purified GST fusions to EHEC virulence proteins separated by PAGE and stained with Coomassie blue. (B) Immunoreactivity of serum from patient 95-8001 against GST-EHEC fusion proteins at days 0, 8, and 60 posthospitalization. (C) Immunoreactivity of patients' sera at 8 days posthospitalization. GST and GST-EHEC fusion proteins were purified as described in Materials and Methods and resolved by SDS–12% PAGE, followed by either staining with Coomassie blue (A) or immunoblotting with patients' sera (B and C). Molecular mass markers (in kilodaltons) are shown to the left of each panel.