Table 1. Clinicopathological evaluation for the reported CSC biomarkers based on TCGA primary HNSCC cohort.

S100 protein family	Neoplasm histologic grade (G1 + G2/G3 + G4), P value	Tumor size (T1 + T2/T3 + T4), P value	Nodal status (N0/N0+), P value	Perineural invasion (no/yes), P value	Pathological nodal extracapsular spread (no/yes), P value	Lympho-vascular invasion (no/yes), P value	New tumor event after initial treatment (no/yes), P value
S100A1	0.676	0.040*a	0.395	0.006*a	0.053	0.018*a	0.758
S100A2	<0.001*b	0.117	0.017*a	0.419	0.061	0.020*b	0.303
S100A3	0.168	0.128	0.167	0.270	0.308	0.264	0.570
S100A4	0.364	0.093	0.410	0.436	0.869	0.838	0.972
S100A5	0.171	0.267	0.436	0.336	0.896	0.282	0.733
S100A6	0.199	0.284	0.341	0.628	0.963	0.332	0.506
S100A7	<0.001*b	0.540	0.013*a	0.244	0.013*b	<0.001*b	0.843
S100A8	<0.001*b	0.147	0.662	0.905	0.254	<0.001*b	0.808
S100A9	<0.001*b	0.208	0.001*b	0.289	0.070	<0.001*b	0.401
S100A10	0.066	0.066	0.110	0.089	0.422	0.901	0.198
S100A11	0.001*b	0.938	0.632	0.478	0.779	0.747	0.168
S100A12	<0.001*b	0.093	0.001*b	0.709	0.001*b	<0.001*b	0.982
S100A13	0.285	0.910	0.058	0.135	0.020	0.149	0.013*a
S100A14	<0.001*b	0.157	0.461	0.289	0.004*b	0.026*b	0.207
S100A16	<0.001*b	0.378	0.011*b	0.234	0.009*b	0.01*b	0.093
S100B	0.047*a	<0.001*b	0.613	0.401	0.477	0.582	0.874
S100P	0.116	0.999	0.126	0.532	0.105	0.464	0.544
S100Z	0.304	0.118	0.532	0.950	0.943	0.695	0.544
S100A7A	<0.001*b	0.569	0.001*b	0.860	0.001*b	<0.001*b	0.295

*, P<0.05; ^a, positive correlation with significance; ^b, negative correlation with significance. CSC, cancer stem cell; TCGA, The Cancer Genome Atlas; HNSCC, head and neck squamous cell carcinoma.