FIG. 1.

In situ hybridization for the detection of hCAP-18 gene expression. (A) Dark-field microscopy showing expression of hCAP-18 in the epithelium of the epididymis. Bright deposit of silver grains indicates hCAP-18 gene expression in the epithelial cells. Magnification, ×24. (B) Bright-field microscopy showing expression of hCAP-18, as detected by black silver grains scattered over the epithelial cells of the epididymis. Magnification, ×39. (C) In the control, an antisense probe yields no labeling of the epithelium in a parallel section of the epididymis. Magnification, ×10. (D) Dark-field microscopy of testis, where no hCAP-18 expression is detected by in situ hybridization. Magnification, ×24.