FIG. 2.

Detection of hCAP-18 protein by immunohistochemistry. Binding of the specific antibody is detected by peroxidase, which yields a dark brown color reaction against a pale background. (A) hCAP-18 is detected in the epithelium of the epididymis (e) but not in the testis (t). Magnification, ×7. (B) Higher magnification of the testis, where no hCAP-18 can be detected by immunohistochemistry. Magnification, ×18. (C) Higher magnification of the epididymis, with strong labeling of the epithelial cells. Spermatozoa and seminal plasma present in the ducts also show the presence of hCAP-18. Magnification, ×29. (D) Control, where the specific antibody has been replaced by nonimmune serum, resulting in loss of labeling. Magnification, ×29.