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. 2022 Nov 15;227(10):1194–1202. doi: 10.1093/infdis/jiac442

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© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Viral population structure. A, Linear map of the RSV genome. B, Phylogenetic tree based on multiple sequence alignment of G protein amino acid sequences. Color indicates amino acids. C, Principal component analysis. PCs 1–3 with labels indicating prolonged infections from different phylogenetic clades. D, A summary of every pairwise genetic distance between every viral sequence is shown (above). Genetic invariance in prolonged infections separated by at least 15 days was compared to other genetic variation within the most closely related sequences (below left) and within all possible closely related pairs (below right). Jitter applied for visualization. Abbreviations: G, glycoprotein; M, matrix protein; PC, principal component; RSV, respiratory syncytial virus; SH, small hydrophobic protein; VE, variance explained.