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. Author manuscript; available in PMC: 2024 May 9.
Published in final edited form as: Immunity. 2023 Feb 14;56(5):1027–1045.e8. doi: 10.1016/j.immuni.2023.01.028

Figure 5. Maturation failure of SMAD4-deficient microglia.

Figure 5.

(A) Strategy used to generate the Smad4 cKO line. Representative confocal image of microglia from either Smad4F/F and Smad4 cKO (n=5 mice/genotype, 4-week-old, two independent experiments).

(B) Cartoon describing the experimental design.

(C) UMAP plot of microglia and BAMs from Smad4F/F and Smad4 cKO littermate mice, split either by cluster or genotype (n=4 mice/genotype, 6–8 weeks old).

(D) Percentage of genotypes in each cluster.

(E) Number and fold-change of differentially expressed genes in brain myeloid populations from Smad4F/F and Smad4 cKO mice.

(F) Heatmap of the top 10 signature genes per cluster.

(G) UMAP plot showing the differential expression of signature genes in Smad4F/F and Smad4 cKO mice.

(H) Representative confocal images of CD206 staining on microglia from Smad4F/F and Smad4 cKO mice (n=5 mice/genotype, 4-week-old, two independent experiments).

(I) UMAP and violin plots showing the expression of Lyve1 and Cd163 in each cluster.

See also Figure S5