Figure 3.

Effects of CDK disruption on cell-cycle phase. (a) Approach for embedding cells such that cell-cycle phases can be measured. In the embedding, the angle Θ indicates phase. (b) Cell-cycle embedding of all MDA-MB-231 cells. (c) Deviation of CDK1 knockout cells from AAVS control cells (grey circle) in density of cells about the cell-cycle embedding (blue). Dashed lines represent the median angle of cell-cycle phases. (d) Deviation in single-cell density compared to AAVS for select knockouts in MDA-MB-231, Hs578T, and MDA-MB-468 cells; *p < 0.05 by Kuiper test.