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. 2023 May 11;10:270. doi: 10.1038/s41597-023-02175-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Results of flow cytometry analysis to estimate the D. kaki genome size. The D. lotus genome (2n = 2x = ~1.76 Gb) served as an internal reference standard. Peak R3 showed DNA amount of D. lotus. Peak R2 showed duplicated DNA amount of D. lotus. The ratio of peak mean was equal 2.52 (R2/R3) and 1.31 (R2/R4), hence the estimated genome size of D. kaki was 2n = 6x = ~4.44 Gb and 4.61 Gb. Due to the distance between peak R2 and peak R4 is less than the distance between peak R2 and peak R3, it is more accurate to estimate genome size of D. kaki 2n = 6x = ~4.61 Gb.