Table 1.
Summary of translation activity of Nluc mRNAs evaluated in this study
| Name of mRNAa | Capping efficiency (%)b | Normalized Nluc expressionc | |||
|---|---|---|---|---|---|
| HeLa cells | JAWS II cells | Mice liver | Mice spleen | ||
| Dinucleotide Cap analogs (m7G-ppp-G) | |||||
| ARCA | 56 | 1.00 ± 0.02 | 1.00 ± 0.03 | n.d. | n.d. |
| ARCA/ AnP | 56 | 0.97 ± 0.03 | 1.13 ± 0.03 | n.d. | n.d. |
| DiPure | >99 | 2.36 ± 0.05 | 3.51 ± 0.09 | n.d. | n.d. |
| DiPure/3′OMe | >99 | 2.27 ± 0.07 | 3.11 ± 0.21 | n.d. | n.d. |
| DiPure/2′OMe | >99 | 2.01 ± 0.09 | 2.58 ± 0.08 | n.d. | n.d. |
| Tri/ tetranucleotide cap analogs m7G-ppp-AG(G) | |||||
| Tri_1 | 87 | 1.00 ± 0.04 | 1.00 ± 0.05 | 1.00 ± 0.15 | 1.00 ± 0.13 |
| Tri_1/ AnP | 87 | 1.07 ± 0.03 | 1.51 ± 0.06 | 1.47 ± 0.23 | 0.61 ± 0.08 |
| Tetra_2 | 52 | 0.52 ± 0.02 | 0.70 ± 0.04 | n.d. | n.d. |
| Tetra_2/AnP | 52 | 0.51 ± 0.02 | 0.53 ± 0.02 | n.d. | n.d. |
| TriPure_0 | >99 | 1.12 ± 0.06 | 1.04 ± 0.08 | n.d. | n.d. |
| TriPure_1 | >99 | 1.31 ± 0.05 | 1.07 ± 0.05 | 0.80 ± 0.08 | 0.90 ± 0.07 |
| TetraPure_2 | 98 | 1.91 ± 0.06 | 2.99 ± 0.19 | 4.86 ± 0.61 | 2.48 ± 0.29 |
| TetraPure_2/m6A | 95 | 1.98 ± 0.07 | 3.60 ± 0.19 | 4.07 ± 0.48 | 3.58 ± 0.63 |
aThe.RNAs were named after the cap analog used. AnP means the RNA was further dephosphorylated using Antarctic phosphatase.
bThese values are taken from Figs. 2f, 4g.
cThese data are calculated from the data shown in Figs. 6c–f, 7, represented as means ± s. e. m. The activities were normalized to the control samples that were ARCA for mRNAs prepared with a dinucleotide cap analog or Tri_1 for mRNAs prepared with a tri/tetranucleotide cap analog. All data were considered for evaluation If the activity was measured at more than one time point.