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. 2023 May 11;14:2350. doi: 10.1038/s41467-023-37824-y

Fig. 3. G-CSF expression is strongly reduced in p140Cap positive BC model.

Fig. 3

a RNASeq Data Analysis was performed on the transcriptomic profiles of mock- vs. p140Cap-expressing TuBo cells. Shown is the list of 18 differentially expressed genes (provided by BIOCARTA) involved in the inflammatory response upon p140Cap expression. b, c p140Cap modulates G-CSF expression and secretion. b G-CSF transcript was analyzed by quantitative real-time RT-PCR in mock and p140Cap TuBo (n = 4) and 4T1 cells (n = 3). G-CSF protein levels were measured in mock and p140Cap TuBo (n = 4) and 4T1 (n = 3) cell culture supernatants by ELISA. Data are represented for n = x experimental repeats and shown as mean ± SEM; two-tailed unpaired t test. c G-CSF transcript levels were analyzed by RT-PCR in mock and p140Cap TuBo and 4T1 tumors. Data are represented for n = 4 mice/group as mean ± SEM; two-tailed unpaired t test.