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. 2023 May 11;14:2350. doi: 10.1038/s41467-023-37824-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Flow cytometry analysis for PMN-MDSCs (CD11b⁺ Ly6G⁺ Ly6C^low), normalized on CD45⁺ cells, in lungs, blood, spleen and bone marrow of tumor-bearing mice. Representative flow cytometry bar plots show: a the percentage of blood, spleen and bone marrow PMN-MDSCs in TuBo (n = 8) and 4T1 (n = 7/blood and bone marrow; n = 9/spleen) mock and p140Cap tumor-bearing mice; (b) the percentage of lung PMN-MDSCs in 4T1 mock and p140Cap tumor-bearing mice (n = 5 mice/group; data are represented for n = x mice as mean ± SEM; two-tailed unpaired t test). c G-CSF protein levels in sera of tumor-bearing mice. G-CSF protein levels were measured in sera collected from TuBo (n = 4) and 4T1 (n = 5) mock and p140Cap tumor-bearing mice by ELISA (Data are represented for n = x mice as mean ± SEM; two-tailed unpaired t test).