Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 11;14:2350. doi: 10.1038/s41467-023-37824-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a, b p140Cap effects on the Tumor-Initiating Cell (TIC) compartment. Mammosphere formation assay from panel (a) mock and p140Cap TuBo (n = 5), 4T1 (n = 5) and MDA-MB-231 (n = 7) cells and from panel (b) mock and p140Cap TuBo tumors (n = 5). Bar plots of mammosphere numbers are shown. Data are represented for n = x experimental repeats and shown as mean ± SEM; two-tailed unpaired t test). c In vivo Limiting Diluition Assay (LDA) of mock and p140Cap TuBo cells. Decreasing amounts (105, 104, 23 and 102) mock and p140Cap TuBo cells were injected into the mammary fat pad of 8-weeks-old female BALB/c mice. Tumor onset was monitored for 5 weeks post-transplantation. The Extreme Limiting Dilution Analysis (ELDA) software was used to calculate the LDA frequencies estimating the number of stem cells in the bulk population in mock and p140Cap TuBo tumors. d, e FACS analysis of stem cell/TIC markers. d FACS analysis of CD44+/CD24− cell populations in mock and p140Cap 4T1 (n = 4) and MDA-MB-231 (n = 3) cells. e FACS analysis of Sca1 expression in TuBo mock and p140Cap cells (n = 5). Bar plots of TIC marker quantification are shown. Data are represented for n = x experimental repeats and shown as mean ± SEM; two-tailed unpaired t test. f GSEA^32,33 plot of WONG_EMBRYONIC_STEM_CELL_CORE in TuBo RNAseq data, and in the CCLE⁵⁶ and TCGA³⁴ database. The plots show the distribution of genes correlated or not with SRCIN1 gene expression; two-tailed unpaired t test. g G-CSF protein levels in mammosphere supernatants. G-CSF protein levels were measured in mock and p140Cap TuBo (n = 4), 4T1 (n = 5) and MDA-MB-231 (n = 4) mammosphere culture supernatants by ELISA. Data are represented for n = x experimental repeats and shown as mean ± SEM; two-tailed unpaired t test. h Bar plot represent the normalization of G-CSF (pg/ml), shown in panel (g), on mammosphere number generated from mock and p140Cap TuBo (n = 4), 4T1 (n = 5) and MDA-MB-231 (n = 4) cells shown in panel (a). Data are represented for n = x experimental repeats, two-tailed unpaired t test.