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. 2023 May 11;381(4):16. doi: 10.1007/s41061-023-00426-9

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Diagram of the flow system used to determination of ACh, where: CS carrier solution (buffer at pH 11), OS extractant (TBPE H solution in dichloroethane), P pump (flow rate equal to 0.8 mL/min), S sample injection (140 µL), RC reaction coil (3 m × 0.5 mm ID), PS phase separator, FC flow cell (8 µL), C circulator, D detector, Rec recorder, NV needle valve, W_aq aqueous waste, W_o organic waste (based on [80, 93])