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IFA analysis of P. yoelii merozoites using double-staining technique. Merozoites in two different fields are shown in the left and right panels. (A) Mab302 culture supernatant, used directly and visualized with FITC-labeled anti-mouse IgG. (B) Purified rabbit anti-PfP0N IgG solution, 1 mg/ml, used at a 1:50 dilution and visualized with rhodamine-labeled anti-rabbit IgG. Bar, 1 μm.
